NMR evidence for an acetylcholine ATP complex.

نویسندگان

  • E P Richards
  • R R Sharp
چکیده

INTRODUCTION It has been known for several years that epinephrine is stored with ATP and there have been several NMR studies of this complex (1,2). Until recently the nature of acetylcholine storage vesicles has remained a mystery. It has now been demonstrated that acetylcholine, in some systems, is stored with a specific protein and ATP (3,4). While a previous study failed to show any interactions between acetylcholine and ATP (51, our work has demonstrated the formation of an acetylcholine ATP complex with a definite stoichiometry. We feel that in the light of the recent evidence that acetylcholine is stored with ATP this complex should be of great interest to workers in the field. In order to study the interaction of acetylcholine and ATP Tl's were determined for acetylcholine alone, and in different mole ratios with ATP. Proton relaxation results from magnetic dipole coupling between neighboring spins on the same molecule, and the relaxation rate (T1jmi is directly proportional to the reorientational

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عنوان ژورنال:
  • Biochemical and biophysical research communications

دوره 64 3  شماره 

صفحات  -

تاریخ انتشار 1975